HPLCと冷蒸気原子蛍光分析を用いる生物試料中の水銀種分別定量
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海産食品などの生物試料中に含まれる水銀種について，HPLC，UV 照射による酸化分解，及び冷蒸気原子蛍光分析を組み合わせた方法（HPLC-UV-CVAFS）により分別定量を行った．まず，生物試料中からの水銀種の抽出法について検討し，メルカプト基をもつ化合物である2-メルカプトエタノール（2-ME）が，抽出剤として効果的であることが分かった．CH3Hg＋濃度の認証値をもつ3 種の認証標準物質について，2-ME 溶液による抽出とHPLC-UV-CVAFS により測定を行い，認証値と測定値がほぼ一致することを確認した．マグロ類などの海産食品試料（実試料）について，同様の分析を行った．抽出溶液について，HPLC-UV-CVAFS 測定によって得られるCH3Hg＋濃度とHg2＋濃度の合計値が，加熱気化原子吸光分析（HVAAS）によって得られる値（総水銀濃度）とほぼ一致した．すなわち，海産食品等の生物試料について，2-ME 抽出によって得られる抽出液をHPLC-UV-CVAFS 測定することにより，水銀種の分別定量が効果的に行えることが分かった．The combination of HPLC, oxidative decomposition by UV irradiation, and cold vapor atomic fluorescence spectrometry (HPLC-UV-CVAFS) was applied to the fractionaldetermination of mercury species, which were contained in biological samples, such as seafood. Firstly, the extraction of mercury species from biological samples was studied, and it was found that 2-mercaptoethanol (2-ME), which has a mercapto group, was an effective extractant. For three certified reference materials, which have the certified values of the CH3Hg＋concentration, extraction by a 2-ME solution and the HPLC-UV-CVAFS measurement werecarried out. It was confirmed that the measured values were about the same as the certified values. The same analytical method was applied to real seafood samples. The sum of the CH3Hg＋ concentration and the Hg2＋ concentration in the extraction solution, which was obtained by the HPLC-UV-CVAFS measurement, was about the same as the total mercury concentration obtained by the heat vaporization atomic absorption spectrometry (HVAAS) measurement. Therefore, it is evident that the fractional determination of mercury species in biological samples was favorably performed when the extraction solution obtained by the 2-ME extraction was analyzed by HPLC-UV-CVAFS.
- 公益社団法人日本分析化学会の論文
公益社団法人日本分析化学会 | 論文
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