Effect of topically applied sphingomyelin-based liposomes on the ceramide level in a three dimensional cultured human skin model

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Sphingomyelin-based liposomes were prepared and applied to the stratum corneum side or basal layer side of a three-dimensional cultured human skin model, and the increase in the type II ceramide (ceramide II) content of the cultured skin model was evaluated. The sphingomyelin-based liposomes were prepared by a high-pressure emulsification method, and the obtained liposomes were characterized; the particle diameter and zeta potential of the liposomes were 155.3 nm and -11.4 mV, respectively. Their spherical shape and lamella structure were observed by transmission electron microscopy. The sphingomyelin-based liposomes or saline were applied to the cultured skin model, and ceramide II was extracted from the skin model. The extracted ceramide II was separated by HPTLC and quantified by a densitometer. The amount of ceramide II in the cultured skin model was significantly increased by the application of the sphingomyelin-based liposomes compared with the non-application group. Thus, sphingomyelin-based liposomes are useful for enriching theceramide level in three dimensional cultured skin models.
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