RT-PCRと細胞培養法を組合せた伝染性造血器壊死症ウイルス(IHNV)の検出
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概要
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Direct RT-PCR, tissue culture and RT-PCR combined with tissue culture were compared for the IHNV inspection in juvenile masu (Oncorhynchus masou) and sockeye salmon (O. nerka). The detection rate of IHNV from artificially infected rainbow trout (O. mykiss) was also compared by using tissue culture and RT-PCR combined with tissue culture. IHNV-infected salmonid fish were detected highly using RT-PCR combined with tissue culture. This method restrained inhibition of IHNV RNA extraction, because IHNV was replicated in the inoculated cells and inhibition of IHNV RNA extraction was diluted. The RT-PCR combined with tissue culture is a good method for the routine examination of IHNV.
- 水産庁さけ・ます資源管理センターの論文
- 1998-12-00
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- RT-PCRと細胞培養法を組合せた伝染性造血器壊死症ウイルス(IHNV)の検出