Limited inhibitory effects of non-steroidal antiinflammatory drugs on in vitro osteogenic differentiation in canine cells
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Cyclooxygenase (COX)-2 participates essentially in bone healing, demonstrated by COX-2knockout mice that showed delayed fracture repair. Considerable controversy still existson inhibitory effects of COX-2 inhibitors on bone healing in clinical cases. To assess stagedependenteffects of short-term treatment of COX-2 inhibitors on osteogenic differentiation,a canine POS osteosarcoma cell line which spontaneously differentiates into osteoblasticcell was exposed to COX-2 inhibitors such as carprofen and meloxicam for 72 hours duringthree different stages of osteoblast differentiation, including day 0 to 3 (pre-osteoblasticstage), day 4 to 7 (transitional stage) and day 8 to 11 (mature osteoblastic stage). Asosteogenic markers, expression of alkaline phosphatase (ALP) was estimated by analysis ofmRNA expression, enzymatic activity and ALP staining, and expression of osteocalcin wasestimated by analysis of mRNA expression after the drug treatments. Calcified matrixformation was finally observed by von Kossa staining on day 14. Expressions of ALPshowed no significant suppression by carprofen and meloxicam during all three stages.However, expressions of osteocalcin mRNA and non-calcified nodule formations weredelayed by carprofen and meloxicam during transitional stage. Nevertheless, fully calcifiednodule formation was observed in all experimental groups during post-medication period.These results indicate that short-term treatment of carprofen and meloxicam wouldreversibly suppress the differentiation of osteoblasts.
- Graduate School of Veterinary Medicine, Hokkaido Universityの論文
- 2013-08-00
Graduate School of Veterinary Medicine, Hokkaido University | 論文
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