A horizontally transferred tRNACys gene in the sugar beet mitochondrial genome: evidence that the gene is present in diverse angiosperms and its transcript is aminoacylated
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Of the two tRNACys(GCA) genes, trnC1-GCA and trnC2-GCA, previously identified inmitochondrial genome of sugar beet, the former is a native gene and probably a pseudocopy, whereas the latter, of origin unknown, is transcribed into a tRNA [tRNACys2(GCA)].In this study, the trnC2-GCA sequence was mined from various public databases. Toevaluate whether or not the trnC2-GCA sequence is located in the mitochondrial genome,the relative copy number of its sequence was assessed in a number of angiosperm species,using a quantitative real time PCR assay. The trnC2-GCA sequence was found to existsporadically in the mitochondrial genomes of a wide range of angiosperms. Themitochondrial tRNACys2(GCA) species from sugar beet, spinach and cucumber werefound to be aminoacylated, indicating that they may participate in translation. We alsoidentified a sugar beet nuclear gene that encodes cysteinyl-tRNA synthetase, which isdual-targeted to mitochondria and plastids, and may aminoacylate tRNACys2(GCA). Whatis of particular interest is that trnC1-GCA and trnC2-GCA co-exist in the mitochondrialgenomes of eight diverse angiosperms, including spinach, and that the spinachtRNACys1(GCA) is also aminoacylated. Taken together, our observations lead us tosurmise that trnC2-GCA might have been horizontally transferred to a common ancestorof eudicots, followed by co-existence and dual-expression of trnC1-GCA andtrnC2-GCA in mitochondria with occasional loss or inactivation of either trnC-GCA geneduring evolution.
- Wiley-Blackwellの論文
- 2011-10-00
Wiley-Blackwell | 論文
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