PIPKs are essential for rhizoid elongation and caulonemal cell development in the moss Physcomitrella patens

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概要

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• PtdIns-4,5-bisphosphate is a lipid messenger of eukaryotic cells playing critical roles inprocesses such as cytoskeleton organization, intracellular vesicular trafficking, secretion,cell motility, regulation of ion channels and nuclear signalling pathways. The enzymesresponsible for the synthesis of PtdIns(4,5)P2 are phosphatidylinositol phosphate kinases(PIPKs). The moss Physcomitrella patens contains two PIPKs, PpPIPK1 and PpPIPK2. Tostudy their physiological role, both genes were disrupted by targeted homologousrecombination and as a result mutant plants with lower PtdIns(4,5)P2 levels were obtained.A strong phenotype for pipk1, but not for pipk2 single knockout lines, was obtained. Thepipk1 knockout lines were impaired in rhizoid and caulonemal cell elongation, whereaspipk1-2 double knockout lines showed dramatic defects in protonemal and gametophoremorphology manifested by the absence of rapidly elongating caulonemal cells in theprotonemal tissue, leafy gametophores with very short rhizoids, and loss of sporophyteproduction. pipk1 complemented by overexpression of PpPIPK1 fully restored the wildtype phenotype whereas overexpression of the inactive PpPIPK1E885A did not.Overexpression of PpPIPK2 in the pipk1-2 double knockout did not restore the wild typephenotype demonstrating that PpPIPK1 and PpPIPK2 are not functionally redundant. Invivo imaging of the cytoskeleton network revealed that the shortened caulonemal cells inthe pipk1 mutants was the result of the absence of the apicobasal gradient of cortical F-actincables normally observed in wild type caulonemal cells. Our data indicate that bothPpPIPKs play a crucial role in the development of the moss P. patens, and particularly inthe regulation of tip growth.

• Blackwell Publishingの論文

著者

• Mikami Koji

  Faculty Of Fisheries Sciences Hokkaido University

• Saavedra Laura

  Department Of Biochemistry Center For Chemistry And Chemical Engineering Lund University

• Sommarin Marianne

  Umea Univ. Umea Swe

• Sommarin Marianne

  Department Of Biochemistry Center For Chemistry And Chemical Engineering Lund University

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