Prediction of maturational competence of feline oocytes using supravital staining of cumulus cells by propidium iodide

概要

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We examined the relationship between integrityof Cumulus cells and nuclear maturation rate afterin vitro culture to determine a non-invasive prediction of the maturationalcompetence of feline oocytes･Feline cumulus-oocyte complexes (COCs) were collected from either small (400-800l九m) or large(≧800 l⊥m) follicles･ Immediately after collection′ cumulus cells were evaluated morphologically(thickness of cumulus cell layers) and stained with propidium iodide (PI)′ which penetrates only nonviablecells･ Cumulus cells without PI staining were judged as having good membrane integrity･ Afterevaluation, COCs were cultured for 30 h and their nuclear maturation rate was determined. The nuclearmaturation rate of oocytes derived from large follicles (89･8%) was higher (p < 0･05) than that fromsmall follicles (60･8%)･ There was no difference in the maturation rate of oocytes from follicleswith thesame size regardless of cumulus morphology ln contrast′ Oocytes that had cumulus cells with goodmembrane integrity showed a higher mahration rate (93･8%) than oocytes with poor cumulus integrity(76･9%) in large follicles (p < 0･05)･ We conclude that evaluation of membrane integrity of cumulus cellsby propidium iodide stainlng Can be used to predict the maturational competence of oocytes･
Cambridge University Pressの論文