Yeast Methylotrophy and Autophagy in a Methanol-Oscillating Environment on Growing Arabidopsis thaliana Leaves

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微生物（C１酵母）は植物葉上に浸み出したアルコールを夜に飲む～細胞センサーの開発と植物葉上のメタノール変動～. 京都大学プレスリリース. 2011-09-27.The yeast Candida boidinii capable of growth on methanol proliferates and survives on the leaves of Arabidopsis thaliana.The local methanol concentration at the phyllosphere of growing A. thaliana exhibited daily periodicity, and yeast cellsresponded by altering both the expression of methanol-inducible genes and peroxisome proliferation. Even under thesedynamically changing environmental conditions, yeast cells proliferated 3 to 4 times in 11 days. Among the C1-metabolicenzymes, enzymes in the methanol assimilation pathway, but not formaldehyde dissimilation or anti-oxidizing enzymes,were necessary for yeast proliferation at the phyllosphere. Furthermore, both peroxisome assembly and pexophagy, aselective autophagy pathway that degrades peroxisomes, were necessary for phyllospheric proliferation. Thus, the present study sheds light on the life cycle and physiology of yeast in the natural environment at both the molecular and cellular levels.
2011-09-26