Enhanced polyamine accumulation alters carotenoid metabolism at the transcriptional level in tomato fruit over-expressing spermidine synthase
スポンサーリンク
概要
- 論文の詳細を見る
Polyamines are involved in crucial plant physiological events, but their roles in fruit development remain unclear. We generated transgenic tomato plants that show a 1.5- to 2-fold increase in polyamine content by over-expressing the spermidine synthase gene, which encodes a key enzyme for polyamine biosynthesis. Pericarp-columella and placental tissue from transgenic tomato fruits were subjected to 1H-nuclear magnetic resonance (NMR) for untargeted metabolic profiling and high-performance liquid chromatography-diode array detection for carotenoid profiling to determine the effects of high levels of polyamine accumulation on tomato fruit metabolism. A principal component analysis of the quantitative 1H NMR data from immature green to red ripe fruit showed a clear discrimination between developmental stages, especially during ripening. Quantification of 37 metabolites in pericarp-columella and 41 metabolites in placenta tissues revealed distinct metabolic profiles between the wild type and transgenic lines, particularly at the late ripening stages. Notably, the transgenic tomato fruits also showed an increase in carotenoid accumulation, especially in lycopene (1.3- to 2.2-fold), and increased ethylene production (1.2- to 1.6-fold) compared to wild-type fruits. Genes responsible for lycopene biosynthesis, including phytoene synthase, phytoene desaturase, and deoxy-d-xylulose 5-phosphate synthase, were significantly up-regulated in ripe transgenic fruits, whereas genes involved in lycopene degradation, including lycopene-epsilon cyclase and lycopene beta cyclase, were down-regulated in the transgenic fruits compared to the wild type. These results suggest that a high level of accumulation of polyamines in the tomato regulates the steady-state level of transcription of genes responsible for the lycopene metabolic pathway, which results in a higher accumulation of lycopene in the fruit.
論文 | ランダム
- ヒト単球系細胞株UG3はマウス間質細胞株ST2と共存培養することにより破骨細胞様細胞に分化する
- 長期観察できた神経線維腫症II型症例の検討
- 低用量のAraC,VP-16とG-CSFの併用療法中に著名な皮膚浸潤を呈した急性骨髄性白血病(M5a)
- II-167 潰瘍性大腸炎及び家族性大腸腺腫症に対する回腸嚢肛門管吻合術の適応と術後肛門機能(第50回日本消化器外科学会総会)
- 救急医療チームがおさえておきたい診断・治療・予防のポイント どう診る? どう対応する? 乳幼児の頭部外傷と虐待(6)虐待への社会的介入,そして予防--診断は終わりでなく始まり