Purification of Extracellular Laccase from Rhizoctonia praticola

概要

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Rhizoctonia praticola was found to produce large amounts of extracellular laccase when grown aerobically on the optimized Lindenberg and Holm medium in fermenter culture with automatic pH control. The laccase from this source was purified to homogeneity by a rapid procedure, using ion?exchange chromatography, affinity chromatography and chromatofocussing. The enzymes isoforms were recovered with a 38? to 115?fold increase in specific activity and a yield for lac1 = 38.93%; lac2 = 14.71% and lac3 = 7.65%. The molecular weight of the purified enzymes proved to be 215, 175 and 68 kDa respectively as determined by size?exclusion HPLC. The isoelectric points were between 6.6 and 7.8, and the carbohydrate content in the purified enzymes was between 6.4?9.7%.