Identification of a Novel Uromodulin-Like Gene Related to Predator-Induced Bulgy Morph in Anuran Tadpoles by Functional Microarray Analysis

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概要

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• Tadpoles of the anuran species Rana pirica can undergo predator-specific morphological responses. Exposure to a predation threat by larvae of the salamander Hynobius retardatus results in formation of a bulgy body (bulgy morph) with a higher tail. The tadpoles revert to a normal phenotype upon removal of the larval salamander threat. Although predator-induced phenotypic plasticity is of major interest to evolutionary ecologists, the molecular and physiological mechanisms that control this response have yet to be elucidated. In a previous study, we identified various genes that are expressed in the skin of the bulgy morph. However, it proved difficult to determine which of these were key genes in the control of gene expression associated with the bulgy phenotype. Here, we show that a novel gene plays an important role in the phenotypic plasticity producing the bulgy morph. A functional microarray analysis using facial tissue samples of control and bulgy morph tadpoles identified candidate functional genes for predator-specific morphological responses. A larger functional microarray was prepared than in the previous study and used to analyze mRNAs extracted from facial and brain tissues of tadpoles from induction-reversion experiments. We found that a novel uromodulin-like gene, which we name here pirica, was up-regulated and that keratin genes were down-regulated as the period of exposure to larval salamanders increased. Pirica consists of a 1296 bp open reading frame, which is putatively translated into a protein of 432 amino acids. The protein contains a zona pellucida domain similar to that of proteins that function to control water permeability. We found that the gene was expressed in the superficial epidermis of the tadpole skin.

• The Public Library of Science (PLoS)の論文
• 2009-06-16

著者

• Nishimura Kinya

  Graduate School Of Fisheries Sciences Hokkaido University

• KISHIDA Osamu

  Center for Ecological Research, Kyoto University

• Kishida Osamu

  Center For Ecological Research Kyoto University

関連論文

• Coupling of two competitive systems via density dependent migration
• Top-down effects on antagonistic inducible defense and offense
• Geographic variation in a predator-induced defense and its genetic basis
• DIFFERENTIATED REACTION NORMS UNDER DIFFERENT SELECTIONS IN POLYMORPHISM: BROAD-HEADED MORPH IN HYNOBIUS RETARDATUS(Behavior Biology and Ecology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• GENETIC BASIS OF PHENOTYPIC PLASTICITY FOR PREDATOR-INDUCED MORPHOLOGICAL DEFENSES IN ANURAN TADPOLE USING cDNA SUBTRACTION AND MICROARRAY ANALYSIS(Taxonomy and Systematics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Soci
• Identification of a Novel Uromodulin-Like Gene Related to Predator-Induced Bulgy Morph in Anuran Tadpoles by Functional Microarray Analysis
• Inducible defenses in prey intensify predator cannibalism
• Evolutionary ecology of inducible morphological plasticity in predator-prey interaction : toward the practical links with population ecology

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