Differential expression of the auxin primary response gene MASSUGU2/IAA19during tropic responses of Arabidopsis hypocotyls
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概要
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We have examined the expression pattern of an auxin primary response gene, MSG2/IAA19, during photo- and gravitropic responses of hypocotyls using a transgenic Arabidopsis harboring MSG2/IAA19 promoter::GUS. The upper portion of most etiolated hypocotyls showed uniform b-glucuronidase (GUS) staining with the strongest activity in the pericycle. When hypocotyls were irradiated with unilateral blue light, GUS activity on the concave side of hypocotylswas decreased, resulting in differentialGUSstaining with a stronger signal on the convex side. The number of differentially stained hypocotyls peaked at 24 h after the onset of the phototropic stimuli, while hypocotyl curvature continued to increase for the entire 36-h experimental period. This result suggests that the MSG2/IAA19 expression precedes the phototropic responses. When seedlings were grown under dim white light, their hypocotyls displayed almost no GUS activity. The light-grown hypocotyls also showed differential GUS staining after phototropic stimuli as result of the increase in GUS activity on the convex side of hypocotyls, especially in the epidermis, the outer cortex and pericycle, although GUS activity was much weaker than that observed in etiolated hypocotyls. Similar but less obvious differential staining was obtained for gravitropic response of hypocotyls. Considering the recent finding thatAux/IAA proteins are immediate targets of the auxin F box receptors, MSG2/IAA19 is likely to act as one of master genes for tropic responses.
- Blackwell Publishingの論文
- 2007-04-04
著者
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綿引 雅昭
北海道大・地球環境科学・環境分子生物
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綿引 雅昭
北大・理学・形態機能I
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Watahiki Masaaki
Division Of Biological Science Graduate School Of Environmental Earth Science Hokkaido University
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Watahiki Masaaki
Grad. School Environ. Earth Science Hokkaido Univ.
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Watahiki Masaaki
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Watahiki Masaaki
Grad. Sch. Environ. Earth Sci. Hokkaidouniv.
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