Inactivation strategy for pseudorabies virus in milk for production of biopharmaceuticals

概要

論文の詳細を見る
By selecting pseudorabies virus (PrV) as a model virus, this study assessed the feasibilityof applying viral inactivation strategies to manufacturing medicinal products from themilk of transgenic sows. The efficacy of heat, acidic/alkaline and detergent treatments wasalso evaluated with respect to their ability to inactivate PrV in milk samples.Experimental results indicate that PrV was inactivated obviously at least 7.125 log10 for 30min at 60°C. At alkaline values of pH 10 and acidic value of pH 4, PrV infectivity wasreduced to 3.625 log10 and exceeded 5 log10, respectively. Moreover, PrV virus wasinactivated efficiently (＞ 3.875 log10) by using 0.25 ～ 1% of Triton X-100 treatment andwithout a loss of biological activity of the recombinant human coagulation factor IX(rhFIX). Results of this study demonstrate the effectiveness of the proposed detergentinactivation method for PrV inactivation of rhFIX production from transgenic products,especially in milk materials.