Possible Underestimation of ChlorophyII a Measurements for Subtropical Phytoplankton Community by the Pigment Extraction and the Fluorometric Determination

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概要

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• Chlorophyll a concentrations of subtropical phytoplankton community extracting with 90% acetone and N, N-dimethylformamide (DMF) were determined with the acidfield and non-acidfield fluorometric method showed a considerably underestimation than those extracted with DMF and determined with the non-acidified fluorometric measurements. The underestimation was not size-specific but observed for pico-, nano- and micro-phytoplankton. Based on variance analysis (two-way ANOVA), the extraction with 90% acetone and the acidified fluorometric determination are both significant as the underestimating sources although the former was more important. From these results, we recommend combination of pigment extraction with DMF and the non-acidified fluorometric measurement in chlorophyll a determinations for subtropical phytoplankton community.

著者

• KOBARI TORU

  Fisheries Biology and Oceanography Division, Faculty of Fisheries, Kagoshima University

関連論文

• Possible Underestimation of ChlorophyII a Measurements for Subtropical Phytoplankton Community by the Pigment Extraction and the Fluorometric Determination
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