Molecular cloning and structural analyses of the pyrF gene for orotate monophosphate (OMP)decarboxylase from extremely halophilic archaeon Haloarcula japonica strain TR-1

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The orotate monophosphate decarboxylase gene (pyrF) in the pyrimidine biosynthetic pathway have unique characteristics. In this study, the pyrF gene was found to encode a polypetide consisting of 277 amino acid residues, showing 96,0 ％ identity to that of H.marismortui. Southern hybridization of the pyfF gene region with partial sequence probe revealed that single copy number of the gene is distributed on the chromosome of H. japonica genome. By phylogenetic analyses of the H. japonica PyrF, the amino acid sequences of the enzyme is relatively conserved in other archaeal strains including methanogens and thermophiles. Alignments of several sequences containing pyrF gene showed that the genetic organization of pyrF gene was not conserved in several haloarchaeal strains expect for H.marismortui.