Silkworm expression system as a platform technology in life science

スポンサーリンク

概要

• 論文の詳細を見る

• Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for academic and industrial purposes. Several recombinant proteins produced by silkworms have already been commercialized. However, construction of a recombinant baculovirus containing a gene of interest requires tedious and troublesome steps and takes a long time (3-6 months). The recent development of a bacmid, Escherichia coli and Bombyx mori shuttle vector, has eliminated the conventional tedious procedures required to identify and isolate recombinant viruses. Several technical improvements, including a cysteine protease or chitinase deletion bacmid and chaperone-assisted expression and coexpression, have led to significantly increased protein yields and reduced costs for large-scale production. Terminal N-acetyl glucosamine and galactose residues were found in the N-glycan structures produced by silkworms, which are different from those generated by insect cells. Genomic elucidation of silkworm has opened a new chapter in utilization of silkworm. Transgenic silkworm technology provides a stable production of recombinant protein. Baculovirus surface display expression is one of the low-cost approaches toward silkworm larvae-derived recombinant subunit vaccines. The expression of pharmaceutically relevant proteins, including cell/viral surface proteins, membrane proteins, and guanine nucleotide-binding protein (G protein) coupled receptors, using silkworm larvae or cocoons has become very attractive. Silkworm biotechnology is an innovative and easy approach to achieve high protein expression levels and is a very promising platform technology in the field of life science. Like the "Silkroad," we expect that the "Bioroad" from Asia to Europe will be established by the silkworm expression system.

• Springer Berlinの論文

著者

• Maenaka Katsumi

  Med. Inst. Bioreg. Kyushu Univ.

• Maenaka Katsumi

  Div. Of Structural Biology Kyushu Univ.

• Kajikawa Mizuho

  Div. Of Struct. Biol. Med. Inst. Of Bioreg. Kyushu Univ.

• Park Enoch

  Shizuoka Univ. Shizuoka Jpn

関連論文

• 3P-015 MHC分子を構成するβ2ミクログロブリンのNMR構造解析(蛋白質・物性(3),第46回日本生物物理学会年会)
• 1P139 BmNPV expression system; Application to human natural killer cell receptors(4. Protein engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 1P076 Molecular basis for recognition of E-cadherin by killer cell-lectin like receptor, KLRG1(2. Protein function (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 1P064 Comprehensive analysis of the MHCI binding of leukocyte immunoglobulin-like receptors using surface plasmon resonance technique(2. Protein function (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 1P027 Structual and biochemical studies of MHC class I recognition by human Leukocyte Ig-like receptors(1. Protein structure and dynamics (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 2P031 Crystallization of the catalytic subunit of archaeal oligosaccharyltransferase(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 1P088 N-linked protein glycosylation in hyperthermophilic archaeon Pyrococcus furiosus(2. Protein function (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Silkworm expression system as a platform technology in life science
• Molecular Basis for Herpesvirus Entry Mediator Recognition by the Human Immune Inhibitory Receptor CD160 and Its Relationship to the Cosignaling Molecules BTLA and LIGHT

スポンサーリンク