コイ肝細胞培養系を用いた化学物質の内分泌撹乱作用検出法の研究　―農薬など各種化学物質の内分泌撹乱作用の検出ー

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A screening test was carried out for estrogenic　effects of 83 chemicals using carp hepatocyte culture. Bisphenol A showed estrogenic effects on both male and female carp hepatocytes, however 4-nonyphenol showed an estrogenic effect (detected in 2 of 3 measurements) on only female cells. Of the agrochemicals tested,tolclofos-methyl and methoxychlor showed an estrogenic effect on male hepatocytes but methoxychlor did not show the effect on female cells. Furthermore, a screening tes t of anti-estrogenic effects was carried out on76 chemicals. Tributyltin compounds showed a potent inhibition on VTG induction by 17β-estradiol in carp hepatocyte culture. Especially,　the anti-estrogenic effect seemed to be potent in female rather than male carp hepatocytes,suggesting a different response to endocrine disrupting chemicals between male and female carp. These results agree with reports that tributyltin compounds caused an imposex of female shellfish in environment.On the other hand,some of the agrochemicals tested (bitertanol, tricyclazole and so on) showed an anti-estrogenic effect on male carp hepatocytes but not neccessarily on female cells.
2004-11-30