正常人およびリンパ増殖性疾患におけるリンパ球 LDH Isoenzyme に関する研究

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Human peripheral lymphocytes were recently classified into two major fractions, T and B lymphocytes. Subsequent studies have uncovered a number of specific features relevant to respective cell fractions, namely, various surface markers and functions specific to each lymphocyte population. It is known, in addition, that enzyme lactate dehydrogenase also differs somewhat according to lymphocyte subfractions. The following studies were carried out to elucidate LDH isozyme changes in various lymphocyte subfractions and in macrophages under physiological and pathological conditions. The results obtained were as follows. 1) The LDH isozyme pattern of human peripheral lymphocytes obtained by PAGE and agarose gel electrophoresis generally disclosed H subunit predominance which significantly differed from those of granulocyte series. 2) Isozyme patterns of lymphocyte sub fractions, T and B cells separated by rosette forming technique showed different patterns characteristic to each cell fraction, namely, H subunit predominance in T cells and relative increase of M subunit in B cells, respectively. 3) Lymphocytes were found to have lesser LDH activity than neutrophils, but no difference was noted between T and B lymphocytes. 4) T and B lymphocytes isolated either by the method of rosette sedimentation or by the Nylon wool filtration method, showed similar isozyme patterns characteristic to either cell fraction irrespective of the separation method employed. 5) The isozyme patterns of thymic and tonsillar lymphocytes exhibited H and M subunit predominance, respectively, which seemed to reflect the major cell components of each original tissue. 6) The LDH isozymes of macrophages had M subunit predominance with increased LDH 3, 4, 5, fractions and were different from those of lymphocytes. Removal of macrophages by the serum coated plate adhesion method did not cause any significant changes in the LDH isozyme pattern of lymphocytes. 7) Mitogen-stimulated lymphocytes resulted in increased LDH activity. Changes in isozyme patterns of lymphocytes stimulated by T cell mitogen showed increases in the LDH activities of fractions 2, 3, 4 whereas PWM stimulation resulted in increases of LDH 3, 4, 5. 8) The malignant cells of lymphoproliferative disorders showed generally higher LDH activity than normal lymphocytes. The LDH isozyme pattern of malignant cells differed between T cell and B cell malignancies and the LDH isozyme pattern of malignant cells also exhibited close resemblance to lymphocyte populations. The above results clearly indicated that peripheral T and B lymphocytes have characteristic LDH isozyme patterns and that the LDH isozyme patterns of malignant cells in lymphoproliferative disorders also show some similarities characteristic to each cell population whose cellular origins were generally reflected by cell surface markers investigated by various means. Thus the LDH isozyme study system would seem to provide interesting tool for examination of the different lymphocyte subpopulations.
札幌医科大学の論文
1981-08-01

正常人およびリンパ増殖性疾患におけるリンパ球 LDH Isoenzyme に関する研究

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札幌医科大学 | 論文

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