末梢血単球の機能に関する研究 ―T細胞芽球化補助作用について―
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The potentiating role of peripheral blood monocytes in PHA-induced T cell blastogenesis was investigated by the co-culture of T cells with autologous or allogeneic monocytes, and also of T cells mixed with the culture supernatant of monocytes stimulated with Con A-Sepharose. Employing the above-mentioned culture designs, the monocyte function was studied in aged subjects and in patients with cancer, systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). The results obtained were as follows. 1) T cell blastogenesis to PHA was enhanced in proportion to the increase of monocyte counts added, and was more remarkably noticed in the PHA concentration of 3μg/ml than in that of 15μg/ml, a conventional dose. 2) There was on difference in the mitogenic response of T cells between autologous and allogeneic co-culture systems. 3) The monocytes from aged subjects were as equally effective as monocytes from young controls in potentiating the blastogenesis of T cells from young controls, but were significantly less effective in the blastogenesis of T cells from aged subjects. 4) In patients with cancer, SLE and RA, a significant decrease was noted in the potentiating effect of monocytes upon the mitogenic responses of T cells from both the patients and healthy controls. 5) It was demonstrated that the culture supernatant of monocytes stimulated by Con A-Sepharose exerted a potentiating effect on PHA-induced T cell blastogenesis. This effect was found to be lower in monocytes of cancer patients than in those of healthy controls. From these results, the decrease of monocyte function in terms of potentiating the T cell blastogenesis was noted among these diseased states. It was also assumed that there was a slight difference in the potentiating manner of monocytes in these disorders.
- 札幌医科大学の論文
- 1981-04-01
札幌医科大学 | 論文
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