実験的尿毒症における大脳皮質アミノ酸代謝に関する研究 ―とくにグルタミン酸代謝を中心として―
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In order to investigate the role of amino acid metabolism, in uremic encephalopathy, the changes in free amino acids in serum and cerebral cortex were measured in uremic rats. Subsequently the uptake of L-(U-14C) glutamate by cerebral cortical slices and its metabolites were studied. The results obtained were as follows ; 1) Uremic rats developed weakness, tremors, muscular twitchings and stupor along with the chemical changes in the serum characteristic of uremia. 2) In serum obtained from uremic rats, aspartate, glycine, 3-methylhistidine, α-aminoadipic acid, cystine and γ-aminobutyrate (GABA) were significantly increased. On the contrary, glutamate, valine, leucine, lysine, tryptophan and tyrosine were significantly reduced. Tyrosine/phenylalanine ratio, valine/glycine ratio and essential amino acids/nonessential amino acids ratio were also apparently reduced. 3) In uremic cerebral cortex, aspartate, glutamine, glycine, histidine, ornithine, phenylalanine, phosphoethanolamine, taurine, 1-methylhistidine and 3-methylhistidine were significantly increased. Carnosine, glutamate and arginine disclosed a significant reduction. 4) Uremic brain did not demonstrate chemical changes of edema. 5) L-14C-glutamate uptake and incorporation into aspartate, glutamate, glutamine, γ-aminobutyrate and CO2 by cerebral cortical slices were markedly decreased in uremic rats. 6) When compared with the percent activity of each metabolite in the total radioactivity recovered, glutamate and aspartate were decreased. In contrast, however, glutamine, γ-aminobutyrate and CO2 revealed moderately enhanced activities. These findings supported the hypothesis that glutamate utilization by TCA cycle was markedly suppressed, while glutamine synthesis and GABA shunt were relatively intact in uremic brain.
- 札幌医科大学の論文
- 1981-04-01
札幌医科大学 | 論文
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