高γグロブリン血症における末梢血リンパ球機能に関する研究 ―とくにPWM刺激免疫グロブリン産生について―
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概要
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In order to study the mechanism of hypergammaglobulinemia from a point of view of T and B cell functions, the appearance rate of Ig-containing cells and the amount of Ig synthesized after stimulation of pokeweed mitogen (PWM) in the mixed culture of T and B cells from peripheral blood lymphocytes were determined by immunofluorescent antibody technique and solid-phase radioimmunoassay, respectively. Diseases with monoclonal gammopathy and polyclonal hypergammaglobulinemia were studied. 1) In normal control subjects, the appearance rate of PWM-induced Ig-containing cells and concentrations of synthesized Ig showed no significant difference between autologous and allogeneic culture systems. 2) In patients with multiple myeloma (MM), both the appearance rate of the cells and the concentrations of all classes of Ig were significantly lower than those of normal control. T cells were considered to display a suppressive function in a mixed culture of patient-T and normal-B cells and to exert an effect on the function and differentiation of B cells in this disease. 3) In contrast to MM, T cells in patients with essential benign monoclonal gammopathy (BMG) exhibited a less suppressive function. However, a remarkable suppressive effect was observed in T cells from patients with BMG accompanied with cancer, malignant lymphoma or Sjogren syndrome. These findings may indicate the differences of T and B cell functions among clinical types of BMG. 4) As to polyclonal hypergammaglobulinemia, there was an increase in concentrations of synthesized Ig in both autologous and allogeneic culture systems in patients with liver cirrhosis, suggesting a hyperfunction of T and B cells. Similar findings were observed in patients with chronic active hepatitis. 5) T cells in patients with RA exhibited suppressor functions on the appearance rate of Ig-containing cells. In patients with SLE in an active stage, T cells showed a helper function on .Ig synthesis, whereas in the inactive stage B cell functions were found to increase. In polyclonal hypergammaglobulinemia, merely the results of Ig-containing cells and synthesized Ig did not seem relevant directly to the cause of hypergammaglobulinemia. Further studies regarding the relations of these results to surface Ig on B cell and local Ig production in tissue lesions will be required.
- 札幌医科大学の論文
- 1980-04-01
札幌医科大学 | 論文
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