3'-Methyl-4-Dimethylaminoazobenzeneラット肝癌形成過程における血清蛋白の変動解析 I. 血清蛋白とくにSeromucoidおよびHaptoglobinとα-Fetoproteinの対比研究
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During the hepatocarcinogenesis of Wistar male rats induced by feeding with 3'-Methyl-4-Dimethylaminoazobenzene (3'-Me-DAB), changes in serum proteins, especially seromucoid (Smc) and haptoglobin (Hp), and the mechanism involved were intestigated in comparison with alpha-fetoprotein (AFP). 1) In the early stages of first to 5th week after 3'-Me-DAB administration, albumin and α1-globulin decreased while γ-globulin increased. After these hepatoma developed, increases of α1, α2 and β-globulins were noted. 2) Serum Smc and Hp levels varied widely in 2nd and 3rd weeks of the feeding, possibly clue to hepatic damage. Both components increased gradually after 6th week and reached a peak at 15th week and after the tumor developed the levels of these proteins were significantly elevated compared with the control rats. 3) A new device for preparing the specific antisera to rat α1-Smc and Hp was developed and these antisera were used for immunohistological investigations. 4) Comparative studies of serum Smc and Hp levels and immunohistological findings indicated that both components were mainly derived from hepatocytes in noncarcinomatous regions but also partly from hepatoma cells and that the increased synthesis in hepatocytes may be the main source of the elevation of serum levels, In .vitro synthesis of both Smc and Hp by Hochwald's method was confirmed to occur in the culture of both hepatoma and nonmalignant tissues. These findings indicate that some hepatoma cells are able to produce these components but the mechanism of increased serum levels may be different from that of AFP and other fetoproteins.
- 札幌医科大学の論文
- 1980-04-01
札幌医科大学 | 論文
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