Ferritinの生合成および代謝回転に関する研究 第2報 Wheat gern lysate を用いた無細胞合成系によるラット肝 ferritin messenger RNA の翻訳
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It is well known that the synthesis of ferritin is induced by the administration of iron. Several contradictory proposals, however, have been made about the mechanism ot induction. In this paper, poly(A+) rich mRNA were isolated from iron loaded rat livers and were translated in a cell-free system derived from wheat germ in order to quantitate the subcellular ferritin mRNA distribution. The following results were obtained with regard to the mode of iron action. 1. The products programmed by rat liver mRNA in wheat germ lysate were first precipitated with antiferritin antiserum and the precipitates were analyzed by SDS gradient gel electrophoresis. Two peptides corresponding to H- and L-subunits from carrier ferritin were obtained and they were considered to be the primary gene product of ferritin synthesis. 2. The ferritin mRNA on polysomes from iron loaded animals gave approximately twice as much incorporation into ferritin as the mRNA from controls by translation assay, and was not due to the simple mobilization of mRNA from mRNP pool to polysomal pool by iron, because the amount of mRNA in the postribosomal supernatant fraction (mRNP) was also increased by iron. 3. On the subfractionation of the postribosomal supernatant by differential centrifugation, nearly all of the postribosomal ferritin mRNP migrated between 80S and 25S in controls, whereas about 50% of mRNA from mRNP particles sedimented at the fraction of less than 25S in iron loaded livers, suggesting that mRNA from the nuclear precursors was temporarily stored at the small mRNP particles, before being converted into the initiation complex and translated on polysomes. 4. The total increase of ferritin synthesis and preferential indution of L-subunits were not so remarkable as compared with the results of in vivo experiment of our first paper, suggesting that intracellular iron also facilitates the translation efficiency of ferritin mRNA on polysomes and the preferential assembly of L-subunits rather than H-subunits on apoferritin shell formation.
- 札幌医科大学の論文
- 1979-10-01
札幌医科大学 | 論文
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