Effect of Photobacterium damselae subsp. piscicida Extracellular Product on Iron Compounds and Expression of Outer Membrane Proteins

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The relation between the activity of Photobacterium damselae subsp. piscicida extracellular products on iron compounds in terms of iron releasing and the bacterial outer membrane proteins (OMPs) profile expressed after iron uptake was studied in this experiment. In order to achieve this, extracellular products (ECPs) collected from cultures of P. d. subsp. piscicida strain P-3179 grown either with or without iron restriction were incubated in dialysis bags with transferrin, an enriched iron compound, or with yellowtail serum in flasks containing medium broth. The ECPs were able to break both the transferrin and yellowtail serum components into smaller units, releasing iron to the culture medium, After removal of the dialysis bags, newly inoculated bacteria into the culture media grew at a higher rate in the flasks that contained broken transferrin and yellowtail serum than the ones without those components. Correspondingly, analysis of the outer membrane proteins collected from P. d. subsp. piscicida after 24 h incubation period showed stronger antigenicity for the OMPs preparation purified from the culture that contained transferrin and ECPs.
高知大学海洋生物教育研究センターの論文
2005-03-31