Organic cation/carnitine transporter octn2 (slc22a5) is responsible for carnitine transport across apical membranes of small intestinal epithelial cells in mouse
スポンサーリンク
概要
- 論文の詳細を見る
出版者許諾要件により、全文非公開.The organic cation/carnitine transporter OCTN2 is responsible for renal tubular reabsorption of its endogenous substrate, carnitine, although its physiological role in small intestine remains controversial. Here we present direct evidence for a predominant role of OCTN2 in small intestinal absorption of carnitine based on experiments with juvenile visceral steatosis (jvs) mice, which have a hereditary deficiency of the octn2 gene. Uptake of carnitine, assessed with an Ussing-type chamber system, from the apical surface of the small intestine was saturable and higher than that from the basal surface in wildtype mice, whereas carnitine uptake having these characteristics was almost absent in jvs mice. Saturable uptake of carnitine was also confirmed in isolated enterocytes obtained from wild-type mice, and the Km value obtained (∼20 μM) was close to that reported for carnitine uptake by human embryonic kidney 293 cells stably expressing mouse OCTN2 (Slc22a5). The carnitine uptake by enterocytes was decreased in the presence of various types of organic cations, and this inhibition profile was similar to that of mouse OCTN2, whereas uptake of carnitine was quite small and unsaturable in enterocytes obtained from jvs mice. Immunohistochemical and immunoprecipitation analyses suggested colocalization of OCTN2 with PDZK1, an adaptor protein that functionally regulates OCTN2. Immunoelectron microscopy visualized both OCTN2 and PDZK1 in microvilli of absorptive epithelial cells. These findings indicate that OCTN2 is predominantly responsible for the uptake of carnitine from the apical surface of mouse small intestinal epithelial cells, and it may therefore be a promising target for oral delivery of therapeutic agents that are OCTN2 substrates. Copyright © 2006 The American Society for Pharmacology and Experimental Therapeutics.
論文 | ランダム
- 「草枕」--霊の感応と非人情
- 「こころ」の死と倫理--我執との相関
- 『彼岸過迄』試論--「松本の話」の機能と時間構造
- 「行人」の構想と構造--一郎・お貞の位相に関する一考察
- 超高齢者の手術におけるADL(日常生活動作)評価の意義 (あゆみ 超高齢者における手術侵襲--評価とその軽減策)