高速液体クロマトグラフィーによるホスファチジルコリン分子種の分析と定量
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概要
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High-performance liquid chromatographic (HPLC) methods for separation and quantitation of molecular species of 1, 2-diacyl-3-acetylglycerol (DG-acetate) derived from phosphatidylcholine (PC) were studied. The PCs were isolated from rat lung, hydrolyzed with phospholipase C and acetylated with acetic anhydride. The DG-acetates were applied on a reverse phase column, eluted by an iso-cratic solvent and detected by U. V. detection at 205 nm or refractometry (RI) detection. By gas chromatographic analyses of fatty acids and total acyl carbon numbers, main molecular species in each peak were identified. The isocratic HPLC method was useful to separate molecular species of lung PC. It was noted that the quantitation of the molecular species can not be obtained by the U. V. detection, especially in the disaturated and monoenoic species. In contrast, the chromatograms obtained by the RI detection and radioactivity determination of 1, 2-diacyl-3-(?H)acetylglycerols prepared by (?H)acetic anhydride were almost identical. Furthermore, the RI detector responded in the same degree for different authentic DG-acetates. These results indicate that the RI detection is very useful for the quantitation among the molecular species containing disaturated species. The composition of molecular species of PCs from different sources were analyzed, and the contents of 16 : 0/16 : 0 species were determined by this HPLC method using RI detector.
- 札幌医科大学の論文
- 1985-02-01
札幌医科大学 | 論文
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