Detection of Human Cytomegalovirus Immediate Early Gene-Specific mRNA by Reverse Transcription Polymerase Chain Reaction.
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概要
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We have established a method for detection of human cytomegalovirus (HCMV) immediate early gene (IE)-specific mRNA by reverse transcription polymerase chain reaction (RT-PCR). Using the IE-1 specific primer fragments designed for RT-PCR, we have amplified a 232 base-pair fragment which represents IE-1 mRNA transcribed from the IE-1 gene of HCMV AD169 strain. No DNA cross reactivities to other human herpes virus DNAs or human embryonic lung cell (MRC-5 line) DNA were observed. The RT-PCR assay indicated HCMV IE-1-specific mRNA in HCMV-infected MRC-5 cells and in peripheral blood specimens from one of 3 patients examined. The results indicate that RT-PCR will be readily applicable to rapid detection of HCMV mRNA and may be useful for diagnosis of active primary or recurrent HCMV infections.
- 札幌医科大学の論文
- 1993-08-01
札幌医科大学 | 論文
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