メチルコラントレソ誘導線維肉腫細胞へのTNF遺伝子導入と同細胞を用いたBALB/cマウスでの腫瘍形成能の検討

概要

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We investigated methods of transduction of the Tumor necrosis factor (TNF) gene into methylcholanthrene-induced fibrosarcoma cells and the tumorigenicity of the transformants in syngeneic BALB/c mice. Tumors in the mice injected with these transformants were found to be rejected by the hosts. We examined the transfection efficiency of two methods : lipofection and retroviral method. In the former method, no stable transformants were established, although some transient expression of TNF was detected. Using the latter method, 10 stable transformants producing significant amounts of TNF were obtained. We selected 3 of those transformants producing different amounts of TNF to examine their tumorigenicity in syngeneic mice. They had showed no difference in growth rate or sensitivity to exogenous TNF treatment in vitro, but their growth rates after inoculation into syngeneic mice were significantly different and showed inverse correlation with tumorigenicity and TNF production. Tumors arising from the high TNF producing clones were rejected within 33 days in all mice examind. These findings indicate that these genetically modified tumor cells will provide a new strategy for cancer treatment.
札幌医科大学の論文
1993-04-01