炎症性腸疾患における粘膜局所免疫グロブリンサブクラス含有細胞の分布異常
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概要
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In order to analyze the local immune state in inflammatory bowel disease (IBD), the distribution of IgG-and IgA-subclass containing cells in colonic mucosa from patients with IBD was investigated by immunohistological methods. In addition, the subclass distribution of specific antibody secreting cells obtained from colonic mucosa was determined by enzyme-linked immunospot (ELISPOT) assay. Results were obtained as follows. 1. Increased proportions of IgG1 and IgA1 containing cells were observed in involved mucosa from patients with ulcerative colitis (UC). In Crohn's disease (CD), not only IgG1 and IgA1 containing cells, but also IgG2 containing cells, showed higher proportions. 2. In involved mucosa of UC, the J-chain positivity of IgA containing cells was reduced, suggesting decreased production of polymeric IgA and enhanced production of monomeric IgA. Regarding IgA subclasses, the J-chain positive IgA2 containing cell fraction was markedly decreased and a reciprocal increase of J-chain negative IgA1 containing cell fraction was observed. In CD, as mentioned above, proportions of IgA1 cells were raised. However, J-chain expression was relatively preserved. These results suggest enhanced secondary immune response associated with mucosal damage and impairment of mucosal immunity in IBD. 3. Compared with involved mucosa, uninvolved mucosa of patients with UC showed similar, although less prominent, alterations in the distribution of immunoglobulin (Ig) subclass containing cells. In CD, with respect to IgA containing cells, uninvolved mucosa showed almost the same degree of alterations in the distribution of subclasses and J-chain as involved mucosa. This finding suggests that even the uninvolved mucosa of CD may have a latent change. 4. Involved mucosa of IBD contained increased numbers of IgG-type anti-LPS antibody secreting cells. Especially, an apparent increase of both IgG1-and IgG2-type antibody secreting cells was observed. Although IgA2-type antibody secreting cells were predominant in normal mucosa, IgA1type antibody secreting cells were increased in involved mucosa of IBD. These findings revealed that alterations in the distribution of Ig subclass containing cells in involved mucosa of IBD partly reflect the enhanced production of antibodies to bacterial antigens.
- 札幌医科大学の論文
- 1991-10-01
札幌医科大学 | 論文
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