ラット後根神経節細胞の偽単極化過程における新知見 ―分散培養細胞のビデオ録画による観察―

概要

論文の詳細を見る
It is well known that dorsal root ganglion (DRG) cells at early stages of develop- ment are bipolar and later become pseudounipolar in the process of cell differentiation. This mor- phological change has also been reported in tissue cultures. The author, using a video recording system, cultured DRG cells on day 18 of gestation and observed the morphological change of the neurons from being bipolar to pseudounipolar. The original process of the DRG neurons first disappeared within 30 minutes in culture and then the neurons elongated forming one or two new neurites. Successive observations of the DRG cells revealed that they were spindle-shaped at 21 hours of culture, eccentric bulged at 23 hours, and bell-shaped at 30 hours, finally becoming short- stem unipolar cells at 34 hours of culture. The elongation of new processes by the unipolarized DRG cell after 48 hours of culture and the change of the DRG cells again into bipolar cells revealed a similarity to the usual unipolarization. The author presumes that DRG cells in the early develop- mental stages of culture repeat the morphological change from bipolar cells to unipolar cells and finally the DRG cells become mature pseudounipolar neurons after two weeks of culture.
札幌医科大学の論文
1991-08-01