癌遺伝子によるマウス骨芽細胞株MC3T3-E1のトランスフォーメーショソ
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概要
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Several oncogenes were introduced into a normal mouse osteoblastic cell line, MC3T3- El, by calcium phosphate-mediated DNA transfection. Transformed foci were formed by cotransfection with adenovirus type 12 E1A (Adl2E1A) and activated c-Ha-ras (EJras) oncogenes, and with c-myc and EJras oncogenes. Transfection with c- myc, Ad12E1A or EJras induced no obvious foci. Transformed cell lines established from each trans- formed focus had high saturation densities in culture and were tumorigenic when injected into athy- mic mice with low-differetiated sarcoma. The transfected oncogene DNA sequences were detected in the cellular DNA of all the transformants. Alkaline phosphatase activity decreased in transformants compared to their parental MC3T3-E1 cells.
- 札幌医科大学の論文
- 1991-06-01
札幌医科大学 | 論文
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