細胞癌化に伴う70kd熱ショック蛋白様分子の細胞表面への構成的発現と,その腫瘍抗原としての意義
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A WKA rat fetus fibroblast (WFB) was transfected by several oncogenes including EJras (activated H-ras), polyoma middle T (PyMT), v-src, c-myc, and adenovirus type 12 E1A-E1B (E1). We analyzed the expression of the transformation-associated cell surface antigens (TAAs) on WFB by developing monoclonal antibodies. One of the WFB TAAs, recognized by monoclonal antibody 067, was constitutively expressed only on two (W31 and W70) of five WFB-EJras-transformed clones. This antigen could not be detected on parental WFB cells nor on as five WFB-PyMT transformant clones. Furthermore, it was not expressed on several clones of partially transformed WFB-v-src nor WFB-adeno E1 transfectants nor on non-transformed WFB-c-myc transfectants. Monoclonal antibody 067 could form an immunoprecipitate with an approximate molecular weight of 67,000 which seemed to be composed of a single polypeptide chain. It was also shown that the expression of this antigen could be enhanced by cyclic AMP or cholera toxin treatment of W31. Moreover, the expression of this antigen could be induced on the WFB-EJ-ras transformants such as W14, which do not constitutively express this antigen. Furthermore, the expression of antigen was enhanced by heat and superoxide treatment on W31. These data suggest that the monoclonal antibody 067-defined molecule is a novel transformation-associated cell surface antigen that could be induced by heat shock or other physiological stress
- 札幌医科大学の論文
- 1990-12-01
札幌医科大学 | 論文
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