合成peptideを用いた抗carcinoembryonic antigen (CEA) モノクローナル抗体の作製とCEA上の抗原決定基の解析
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概要
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The monoclonal antibodies to a synthetic peptide of domain I (119-140 amino acids, P1-peptide) of carcinoembryonic antigen (CEA) were prepared and characterized, in order to analyze the epitope structure of CEA. Antibodies were screened by use of radioimmunoassay employing the P1-peptide as an antigen. The resulting monoclonal antibodies were divided into two groups. Group 1 (SC1) reacted with the synthetic peptide, but not with native CEA preparation. Group 2 (SC2 and SC3) reacted with either type of antigens. Interestingly, after treatment of CEA with periodate, group 1 showed reactivity, suggesting that the amino acid sequence recognized with the monoclonal antibody of group 1 was spatially hidden by carbohydrate residue. These results were confirmed by Western blot analysis and by immunohistochemical method. The monoclonal antibodies of group 2 showed a similar staining pattern to the conventional anti-CEA MoAbs. However, the epitopes recognized by the antibodies of group 2, seem different from the epitope recognized by the conventional MoAbs, when the reactivity patterns of these antibodies against P1-peptide are compared. The amino acid sequences of the epitopes can possibly be speculated from the reactivity of each antibody to the recombinant domains and from the result obtained by blocking assay. These monoclonal antibodies would be useful for further analysis of the epitopes and as a possible diagnostic and therapeutic tool for cancer.
- 札幌医科大学の論文
- 1989-10-01
札幌医科大学 | 論文
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