抗HLA－A2.A28モノクローナル抗体に対する抗イディオタイプモノクローナル抗体の作製と免疫応答の解析

概要

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Model experiments using anti HLA monoclonal antibodies were performed to apply the immune response of anti-idiotypic antibodies to immunotherapy since anti-idiotypic antibodies are of interest in the investigation of a new immunomodulator for cancer patients.Polyclonal anti-idiotypic antisera and anti-idiotypic monoclonal antibodies induced with anti HLA-A2.A28 monoclonal antibody KS1 in a syngeneic system recognized the private idiotype on monoclonal amtibody KS1.Idiotype mapping of monoclonal antibody ES1 showed that there might be at least four idiotopes on it ; two idiotopes at a combining site and another at an intermediate site.The four diffrerent anti-idiotypic monoclonal antibodies might induce anti-anti-idiotypic antibodies specific to their immunized anti-idiotypic monoclonal antibody in the syngeneic system.One of the anti-idiotypic monoclonal antibodies against the idiotope at the combining site of monoclonal antibody KS1could induce an anti-anti-idiotypic antibody which might have the same reactivity as that of monoclonal antibody KS1 to HLA-A2.A28 antigen.The results suggest that an anti-idiotypic antibody bearing the internal image of a tumor associated antigen may induce the anti-anti-idiotypic antibodies specific to the tumor associated antigen and therefore may be useful for cancer therapy.
札幌医科大学の論文
1989-02-01