ヒト子宮頸部腺癌培養細胞株(CAC-1)の樹立とその性状および各種抗癌剤に対する感受性の検討
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A new cell line of a human uterine cervical adenocarcinoma, designated as CAC-1, was established from a moderately differentiated adenocarcinoma of the uterine cervix. CAC-1 was subcultivated more than 150 times during 36 month period. The population doubling time was 31 hours. The number of chromosomes in the majority of cells was hypertriploidy with a mode of 75 at passage 51. In the cytoplasm, alcian blue, PAS and CEA staining positive substances could be seen. The cells produced CA-125, hCG and TPA in cultured media. The tumor obtained from nude mice inoculated with CAC-1 cells was a moderately differentiated adenocarcinoma histologically, closely resembling the original human tumor. CAC-1 was studied for chemosensitivity against 12 anticancer drugs by in vitro colony forming assay. In vitro sensitivity was defined as a 70% or greater inhibition of colony formation when compared to controls. Etoposide, mitomycin C and cisplatin showed sensitivity at peak plasma concentrations for 1 hour exposure. The 8 drugs, which showed sensitivity at 1/10 peak plasma concentration for 24 hour exposure, were aclacinomycin, actinomycin D, mitomycin C, etoposide, adriamycin, vincristine, vinblastine and cisplatin.
- 札幌医科大学の論文
- 1988-12-01
札幌医科大学 | 論文
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