子宮頸部微小浸潤癌における浸潤・増殖に関する超微形態学的及び免疫組織化学的研究

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Elucidation of the infiltrating process of cancer cells is particularly important in the understanding of the biological behavior in various types of cancer. At this time, a comparison between carcinoma in situ (CIS) and newly infiltrated microinvasive cancer was carried out. The uterine cervix is a site which allows for the detection of such early cancers as CIS and microinvasive cancer, because exfoliative cytology, colposcopy and punch biopsy are methods which can easily be performed. In the present communication, the authors made the following studies using human materials of the uterine cervix. At first, transmission electron microscopy (TEM) was carried out for the purpose of examining ultrastructural features of the locally infiltrating lesion in the microinvasive cancer. In these observations, many pseudopod-like cytoplasmic protrusions of cancer cells and abundant microfilaments parallel to the direction of the protrusion were seen. In accordance with the disappearance of the part of the basal lamina, many vesicles, 70-90nm in diameter were also seen, suggesting their role in cancer infiltration. Next, in order to elucidate the relationship between the proliferation and infiltration of cancer cells, by means of the identification of cells in the DNA synthetic phase (S phase), bromodeoxyuridine (BrdU), an analogue to thymidine, was taken up by the cancer tissue with an in vitro injection method and stained with the immunoperoxidase method. Distribution of BrdUpositive cells was examined and the labeling index was calculated. BrdUpositive cells were primarily found in the peripheral area of the cancer nest, but not observed in the adjacent cells to the stroma, which are supposed to be subject to a high infiltrating capacity. Labeling index was high for CIS, CIS adjacent to a microinvasive area and microinvasive area in this order. A correlation may be present between infiltrating and proliferating capacity of cancer tissue. Furthermore, using the immunoperoxidase method, fibronectin (FN), which is a component of extracellular matrices and presumably as a resistant factor against cancer cell infiltration in view of its action on cell adhesion, was examined for its distribution around the invasive lesion. As a result, FN has shown its decrease in the transitional area between the cancer nest and the stroma, at the stage of microinvasion.? These studies showed some correlation between the type of microinvasion and population of BrdU-positive cells or FN staining pattern. In the type of finger-like invasion, a marked decrease of FN was seen around the microinvasive area, but BrdU-positive cells were relatively sparse. In contrast, in the type of bulky outgrowth or drop invasion, BrdU-positive cells were numerous, but FN was decreased slightly. Therefore, it is suggested that cancer cells show a different function between the case of infiltration and that of proliferation.
札幌医科大学の論文
1988-02-01

子宮頸部微小浸潤癌における浸潤・増殖に関する超微形態学的及び免疫組織化学的研究

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札幌医科大学 | 論文

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