血漿 α-Human Atrial Natriuretic Peptide の Radioimmunoassay に関する検討とその臨床応用

概要

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Numerous reports have been published on the measurement of plasma levels of human atrial natriuretic peptide (hANP). However, it should be noted that various values of hANP from 6.4 pg/ml to 200 pg/ml in normal subjects have been described in the various studies. Therefore, to further investigate the pathophysiological role of hANP, establishment of a refined procedure for the determination of hANP in plasma seems essential. In this study, a sensitive and specific radioimmunoassay (RIA) method for α-hANP has been developed by using anti-α-hANP antiserum in combination with 125I-hANP. Plasma α-hANP levels were determined in the patients having various hypertensive diseases. Three types of anti-α-hANP antiserum were obtained. From the study of cross-reactivity to hANP fragments, its was suggested that antiserum-1, -2 and -3 are mostly specific to 1-28 hANP, 5-25 hANP and the ring structure, respectively. The estimated values of this hormone were significantly lower in the order of antiserum-1, -2 and -3. High performance liquid chromatographic study showed that various types of fragments of hANP exist in human plasma and antiserum-1 RIA represents mostly the 1-28 hANP. These findings suggested that the highly specific antiserum to 1-28 hANP such as antiserum-1 should be used to estimate the 1-28 hANP levels in human plasma. The dilution curve of extracted plasma was parallel to that of synthetic 1-28 hANP, while that of non-extracted plasma revealed a non-parallel relation to that of synthetic 1-28 hANP. Therefore, the extraction procedure seems to be necessary to measure the plasma hANP level. Furthermore, it was also clarified in this study that antiserum specificity affects the plasma levels of immunoreactive hANP because of the existence of many kinds and many doses of hANP fragments in human plasma. In addition, it was suggested that some non-specific unidentified substances might interfere with RIA of human plasma. Therefore, it should be considered that the difference of specificity of anti-α-hANP antisera is the most important factor in the various plasma hANP levels reported up to the present, in addition to the procedure of plasma sample treatment. In the determination by using antiserum-1, it was revealed that the plasma hANP increased in essential hypertensives and patients with primary aldosteronism, chronic renal failure and malignant hypertension. Regarding the pathophysiolosical significance of increased plasma hANP, it is unlikely that this factor plays an important role in the etiology of essential hypertension or other hypertensive diseases, because the plasma levels of this hormone are elevated in these patients. Therefore, the increase of plasma hANP levels in these patients should be considered as secondary and a compensatory reaction to the elevated blood pressure.
札幌医科大学の論文
1987-08-01

血漿 α-Human Atrial Natriuretic Peptide の Radioimmunoassay に関する検討とその臨床応用

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概要

札幌医科大学 | 論文

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