モノクローナル抗体によるヒトアミロイド関連蛋白の免疫学的解析
スポンサーリンク
概要
- 論文の詳細を見る
Monoclonal antibody AM34 was generated from the fusion of mouse myeloma P3-X63-Ag8.653 cells with splenocytes obtained from a BALB/c mouse that had been immunized with crude amyloid proteins which were extracted from the amyloid kidney of a patient with rheumatoid arthritis. This antibody bound specifically to amyloid deposits of the AA type of formalin-fixed paraffin-embedded sections, but reacted little or not at all with other tissues including sections of patients with primary and AL type amyloidosis. This reaction was not inhibited by the pretreatment of sections with polyclonal anti-human amyloid antibodies to AA protein. A reversed passive hemagglutination assay (RPHA) using sheep red cells coated antibody AM34 showed the presence of a corresponding antigen in the sera from patients with malignant or inflammatory diseases. Sodium-dodecylsulfate polyacrylamide gel electrophoresis and western blot analysis demonstrated that the corresponding antigen consisted of 45,000 and 42,000 dalton molecules under reducing and non-reducing conditions, when crude amyloid proteins and the sera showing higher titers in RPHA were tested. Interestingly, monoclonal antibody AM34 clearly reacted with senile plaques in the brain tissues obtained from two patients with Alzheimer's disease. Therefore, these data suggest that antibody AM34 may recognize a novel protein having a 45,000 and 42,000 dalton molecular weight which is strongly associated with secondary amyloidosis and probably with Alzheimer's disease.
- 札幌医科大学の論文
- 1987-04-01
札幌医科大学 | 論文
- "すきま"の細胞生物学 -細胞間接着装置タイト結合とヒト疾患-
- 人工内耳手術における顔面神経窩の局所解剖学的検討
- 三次元MRIと高分解能CTによる蝸牛の領域分析 -人工内耳適応の術前診断への応用-
- 消化器癌転移の機序の解析と治療への応用
- 学位申請論文 ヒト気管支上皮細胞株Calu-3をモデルとしたconnexin 26による内耳機能調節のメカニズム解析