ヒト膵癌細胞株増殖におけるホルモンの影響とホルモソレセプターとの関連性に関する基礎的研究

概要

論文の詳細を見る
Three kinds of established human pancreatic cancer cell lines (HPC-1, 3 and 4) cultured in vitro were supplemented with various concentrations of peptide hormones, i.e. secretin, glucagon, caerulein and gastrin, and of steroid hormones, i.e. estrogen, testosterone and hydrocortisone. Effects of these hormones on the cell proliferation and secretion of tumor markers in the media were examined. Presence of the receptors for those hormones was investigated in each cell line. The results obtained were as follows: 1) The increase in cell number and 3H-thymidine incorporation was facilitated in HPC-1 cells by caerulein and estrogen, in HPC-3 cells by secretin, glucagon, caerulein, gastrin and estrogen, and in HPC-4 cells by estrogen. Under 10-4M concentration of steroid hormones, estrogen showed cytotoxicity whereas testosterone and hydrocortisone resulted in the inhibition of proliferation in all cell lines. The inhibitory effect was more significant in testosterone. 2) Measurement of tumor markers secreted in the media was performed on beta2-microglobulin, pancreatic secretory trypsin inhibitor, CEA, CA19-9 and HC-2, a monoclonal antibody produced by using HGC-25 cells. Beta2-microglobulin and HC-2 were secreted by three cell lines, but the amount was parallel with the cell number, suggesting that the hormones did not have a significant influence on tumor marker secretion. 3) Presence of hormone receptors was assessed in each cell line, on the basis of the change of cyclic AMP or cyclic GMP level after hormone administration. It was suggested that there were receptors for caerulein in HPC-1, and those for secretin, glucagon, caerulein and gastrin in HPC-3. The existence of receptors for estrogen and testosterone was indicated by radioreceptor assay of steroid hormones in all cell lines.
札幌医科大学の論文
1987-04-01

ヒト膵癌細胞株増殖におけるホルモンの影響とホルモソレセプターとの関連性に関する基礎的研究

スポンサーリンク

概要

札幌医科大学 | 論文

スポンサーリンク