幼若期水頭症ラットにおける脳組織及び血管床の変化 ―先天性水頭症(LEW/Jms)と幼若期カオリン水頭症における研究―
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概要
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Brain tissue damages and vascular changes were studied using light and electron microscopes in congenital rat hydrocephalus (LEW/Jms) and infantile kaolin-induced hydrocephalus. In this study, 40 congenital hydrocephalic rats (LEW/Jms) and 132 rats injected with kaolin suspension 2 days after birth were examined. For the light microscopic study, rats' brains in both groups were perfused with 10% formalin followed by carbon black infusion. For the electron microscopic study, rats' brains in both groups were perfused with Karnovsky's solution. Samples were refixed in a 1% osmium tetroxide solution, dehydrated with ethanol and embedded in Spurr resin. In light microscopic study of congenital hydrocephalus, there were disruptions of the ependymal layer and local edema formation in the periventricular white matter of the lateral parts of the anterior and posterior horns, which resulted in the formation of an intracerebral cavity. Stenotic or obstructive blood vessels without filling of carbon black were observed in the periventricular white matter. In light microscopic study of kaolin-induced hydrocephalus, edema was diffusely observed in the subependymal white matter. The intracerebral cavity was also observed in the same region as congenital hydrocephalus. Stenosis or obstruction of the blood vessels were focally observed in the periventricular white matter. The difference of the edema formations in the two hydrocephalic models may be due to the difference of immaturation of the infantile brain tissue. In electron microscopic study for these two hydrocephalic models, the enlargement of the extracellular space was noticed in the periventricular edematous region. The endothelial cells of capillaries and venules showed an irregular surface with indentations and contained numerous hollow vesicles. Only in the congenital hydrocephalus, occluded capillaries were found in the border zone between the edematous and non-edematous area. Around the occluded capillaries, there were swellings with a degeneration of the astrocytes and shrinkage of the pericyte. These microcirculatory disturbances of the brain tissue were considered to be one of the etiological factors for the enlargement of the ventricles and intracerebral cavity formation in infantile hydrocephalus.
- 札幌医科大学の論文
- 1986-02-01
札幌医科大学 | 論文
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