p53 Point Mutations in Fine-Needle Aspirated Breast Cancer Cells and Their DNA Ploidy Patterns

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概要

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• Breast cancer cells can be obtained derectly from the patient with minimal damage by fine-needle sampling. The method of aspiration biopsy cytology (ABC) by fine-needle aspiration was developed that enabled us to prepare cancer cell nuclei for detection of p53 gene mutation by PCR-SSCP. Fine-needle sam-pling was successfully performed on 49 patients with breast tumor. Thirty-one aspirated specimens (63.3%) produced enough malignant material for assess-ment ; 18 were diagnosed as being cytologically benign. In 49 patients, surgical specimens from the same tumors were examined for DNA aneuploid and S-phase fraction using flow cytometry. Twenty-four of the 31 breast cancer specimens (77.4%) were classified as aneuploid pattern. p53 gene mutations were detected in 15 patients (48.4%) ; all showed point mutations. In this series, no significant correlation was found with respect to the factors such as aneuploidy, p53 muta-tion, and ER in various tumor size or menopausal status. On the contrary, there was a significant relationship between aneuploidy pattern and p53 mutation ; fifteen p53 mutated cancer cell samples showed all aneuploid patterns. Further-more, these 2 factors showed a tendency of high incidence in advanced clinical stage, histologic grade, and tumor size. Thus, taking ABC into consideration, a combined examination of p53 mutation and DNA histograms derived from flow cytometry in fresh tissue samples could be a valuable tool in clinical use for biological evaluation of breast cancer.

• 札幌医科大学の論文
• 1995-00-00

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