Heterogeneity of Human Breast Cancer Cell Clones with respect to Cytotoxic Susceptibility detected by Cytotoxic T-Lymphocytes and Natural Killer Cells
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概要
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Clonal heterogeneity of human breast cancer cells, HMC-1, with respect to the cytotoxic susceptibility against autologous cytotoxic T-lymphocytes (CTL), TcHMC-1, and natural killer- (NK) cells was demonstrated in a ??Cr release cytotoxicity assay. We have established 8 tumor cell clones, HMC-1-1 through HMC-1-8, from HMC-1 cells and autologous TcHMC-1 clone that showed high cytotoxic activity as well. In the cytotoxicity assays, HMC-1-8 clone showed significantly high cytotoxic susceptibility by TcHMC-1, and conversely, HMC-1-7 clone showed low cytotoxic susceptibility. In case of autologous NK cells as effector, HMC-1-8 clone showed low cytotoxic susceptibility and HMC-1-7 clone did high. On the other hand, as to lymphokine-activated killer (LAK) cells, such heterogeneity as TcHMC-1 and NK cells demonstrated was not shown by either autologous or allogeneic LAK cells. These heterogeneous cytotoxic susceptibilities were further examined to determine whether they are related to the tumorigenicity of HMC-1 clones using a soft agar assay, showing that HMC-1-8 clone indicated significant high plating efficiency and HMC-1-7 showed low plating efficiency. The results of this study indicated that the heter-ogeneity of HMC-1 clones in cytotoxic susceptibility was shown by autologous CTL and NK cells but not by LAK cells, and the NK susceptibility alone seemed to be concerned with the tumorigenicity of tumor cells. To achieve moreeffective cancer immunotherapy, it is thought to be necessary to clarify such a new type of tumor heterogeneity in cytotoxic susceptibility.
- 札幌医科大学の論文
- 1991-00-00
札幌医科大学 | 論文
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