Vaccination with autoreactive CD4+Th1 clones in lupus-prone MRL/Mp-Faslpr/lpr mice
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We studied the efficacy of T cell vaccination with CD4+αβTh1 clones in lupus-prone MRL/Mp-Faslpr/lpr (MRL/lpr) mice. CD4+αβ Th1 clones, dna51 (Vβ8.3) and rnp2 (Vβ14), which stimulated anti-dsDNA or U1 ribonucleoprotein (U1RNP) antibody (Ab) production respectively, were isolated from splenocytes of MRL/lpr mice. Antinuclear Ab kinetics, renal function, renal histology, survival rate, and lymphocyte subpopulations in the spleen were monitored after intravenous adoptive transfer of IL-2-stimulated (s-) or irradiated (i-) clones to 3 week-old female MRL/lpr mice. Anti-idiotypic humoral and T cell responses against the transferred autoreactive Th1 clones were determined in parallel. Compared with PBS-treated MRL/lpr mice, anti-dsDNA Ab titers, and the activity index for lupus nephritis were all decreased in MRL/lpr mice vaccinated with i-dna51 cells, whereas survival rate was not improved. The numbers of CD4+Vβ8.3 T cells in the spleen were also significantly decreased in these mice. Anti-idiotypic Abs recognizing a 12 amino acid sequence of clone dna51 T cell receptor Vβ8.3-complementarity-determining region (CDR) 3 were detected in the MRL/lpr mice that received i-dna51 or s-dna51 cells. These Abs suppressed dna51 cell proliferation, as well as cytotoxicity of CD8+T cells against dna51. The present study suggests that vaccination with CD4+αβTh1 clone, dna51, elicits anti-idiotypic T cell and humoral responses against dna51 in MRL/lpr mice, although the immunoregulatory effects on lupus may be limited.
論文 | ランダム
- 都市の歴史社会学と都市社会学の学問構造(歴史社会学)
- 都市の無計画と過剰計画--街並みの自由と建築物の施設規制 (特集1 持続可能な都市再生)
- 身体と環境の歴史社会学(B編 身体と公共の歴史社会学,都市・公共・身体の歴史社会学-都市社会学誕生100年記念-)
- P.ゲデスと都市社会学の展開(第I部 社会学研究の変貌とゲデスの発見,A編 ゲデス・プロジェクト,都市・公共・身体の歴史社会学-都市社会学誕生100年記念-)
- 近代化と社会学像の変貌--社会学研究の発展と融解 (特集 北東アジアの社会理論--グローバル化時代におけるアジアの社会学理論を中心に(1))