Direct fluorometric detection of paramagnetic and heavy metal ions at sub-amol level using an aromatic polyaminocarboxylate by CZE: Combination of pre- and on-capillary complexation Technique
スポンサーリンク
概要
- 論文の詳細を見る
Copyright(c)2007 John Wiley & Sons LtdThe low sensitivity of simple CZE for detecting metal ions is a long-standing problem even when an LIF detection system is employed. We have successfully achieved an ultrasensitive CE-LIF using a simple CZE mode (typical detection limit: 10-11-10-10 mol/dm3). Both the design of a newly synthesized ligand and the combination of a precapillary derivatizing technique with an on-capillary ternary complexing technique have enabled us to achieve this extremely low LOD and high resolution of large metal complexes. The direct fluorescent detection of the paramagnetic metal ions was achieved for the first time despite their intrinsic fluorescent quenching nature. The fluorescent ligand (L) consists of a polyaminocarboxylate chelating moiety, a strongly emissive fluorescein moiety and a spacer connecting the two portions. The migration behavior of various metal-L complexes was investigated. The resolution among the complexes was improved by the introduction of a ternary complex equilibrium of the kinetically stable mother complexes with OH- ion. The analytical potential of our simple system was examined, and it was proved that the system was one of the most sensitive methods without the need for any preconcentration process.
論文 | ランダム
- 高分子量ポリエステル樹脂に関する研究(第7報)末端基種類と金属酸化物,金属水酸化物の併用による増粘効果
- 高分子量ポリエステル樹脂に関する研究-第5報-分子量とその物性
- 生分解性プラスチックについて
- 不飽和ポリエステル樹脂 (高性能・高機能めざす熱硬化プラスチック--どこまで特殊化できるか)
- 大発生するカゲロウ (特集 水生昆虫)