器官培養系での表皮キヤロンの細胞分裂およびDNA合成に対する効果〔英文〕

概要

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When a piece of mouse ear conch was laid on a disk of glass filter immersed in aTC 199 medium and kept at 37°C in an atmosphere of 95 % O2+5 % CO2 gas mixture, anabrupt increase in the number of mitoses and DNA synthesizing cells was observed between12 and 24 h after the initiation of culture. The skin extract containing epidermalchalone was added to the culture medium at the time on the course of increase in mitosesand DNA synthesizing cells. In the case of DNA synthesizing cells 3H-thymidine wasadded various times later. Both G2-chalone which inhibited the transition from G2 toM and G1-chalone which inhibited the transition from G1 to S required adrenaline ascofactor.A 80 % ethanol precipitate of crude skin extract was fractionated through SephadexG-100 column chromatography. The activity of G2-chalone existed in the low molecularweight fraction (10, 000<MW<100, 000) and that of G1-chalone existed in the high molecularweight fraction (MW>100, 000).
岩手大学教育学部の論文