サイレ-ジより分離した糸状菌の代謝について

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In order to investigate the influence of silage moulds on aerobic deterioration, the chemical changes brought about in the silage extract media with various levels of initial pH by the moulds isolated from corn silage were studied. The silage extract media consisted of silage juice plus pulp cellulose powder and the dry matter contents of the media were adjusted to about 17% which was the same level as corn silage by adding pulp cellulose powder and the media were prepared in cottonplugged culture tubes. Moreover, initial pH values of these media were adjusted to 4, 4.5, 5, 5.5, 6, 6.5 and 7 with 2.5N NaOH or HCl prior to autoclaving, respectively. Three genera of moulds selected were Aspergillus sp. (A. niger), Penicillium sp. (P. roqueforti) and Mucor sp.. Each culture of the moulds was diluted with sterile Ringer's solution and introduced into the medium to give initial counts of 103/10g fresh matter for Aspergillus sp. , Penicillium sp. and Mucor sp. , respectively. The cultures were incubated at 30℃ and the changes in chemical composition of cultures at 0, 2, 4, 6, 8, 10 and 14 days after incubation were measured, respectively. Large increases in pH at 8 days in the cultures of Mucor sp. were found in the higher initial pH values (range of 5.5-7.0), whereas there was a rapid decrease in pH against the lower lactic acid content in the case of Aspergillus sp.. This is probably due to the production of citric acid by Aspergillus sp. used. In all of the cultures of Penicillium sp. pH values markedly increased with time. In spite of initial pH values, lactic acid contents in the cultures of three genera of moulds decreased with time. In the cultures of Penicillium and Mucor sp. the ratios of VBN/TN fairly increased with time, but in the cultures of Aspergillus sp. the ratios decreased in the cases of higher initial pH values (range of 6.0-7.0). Total VFA contents in the cultures of three genera of moulds remarkably increased in the case of lower initial pH values (range of 4.0-5.5). Acetic, iso-butyric and butyric acids were found in all of the cultures. The WSC contents in the cultures decreased immediately after incubation in all cases. And then, the different trends were observed on the changes in WSC contents with time among genera of the moulds and initial pH values of media.
信州大学農学部の論文

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唐沢豊
信州大大学院

サイレ-ジより分離した糸状菌の代謝について

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