三次元培養皮膚を用いた衣服の安全性試験法

概要

論文の詳細を見る
To establish a safety examination method for the clothes that are used in contact with human skin, verification of the experimental conditions was conducted in conformity with Japanese Industrial Standard, using a three-dimensional cultured skin model. It was revealed that the density of cationic surfactants adsorbed on a cloth was correlated closely with the cell viability, suggesting that this method is applicable to assess the primary skin irritation. To quantify the irritant material shifted from a cloth toward the skin a reasonable addition of the irritant material has to be prescribed in the primary skin irritation examination. A suitable dose was found to be in the range of 50-100 μl. Safety examinations of wearing clothes involve an addition of suitable artificial sweat, but the actual sweat contains free fatty acids. So it will be necessary to consider the constituents of the artificial sweat in addition to the dose of the irritant materials.
奈良教育大学の論文
2007-10-31