Roles of Three Domains of Tetrahymena eEF1Ain Bundling F-actin

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概要

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• The conventional role of eukaryotic elongation factor 1A (eEF1A) is to transport aminoacyl tRNA to the A site of ribosomes during the peptide elongation phase of protein synthesis. eEF1A also is involved in regulating the dynamics of microtubules and actin filaments in cytoplasm. In Tetrahymena, eEF1A forms homodimers and bundles F-actin. Ca2+/calmodulin (CaM) causes reversion of the eEF1A dimer to the monomer, which loosens F-actin bundling, and then Ca2+/CaM/eEF1A monomer complexes dissociate from F-actin. eEF1A consists of three domains in all eukaryotic species, but the individual roles of the Tetrahymena eEF1A domains in bundling F-actin are unknown. In this study, we investigated the interaction of each domain with F-actin, recombinant Tetrahymena CaM, and eEF1A itself in vitro, using three glutathione-S-transferase-domain fusion proteins (GST-dm1, -2, and -3). We found that only GST-dm3 bound to F-actin and influences dimer formation, but that all three domains bound to Tetrahymena CaM in a Ca2+-dependent manner. The critical Ca2+ concentration for binding among three domains of eEF1A and CaM were ≤100 nM for domain 1, 100 nM to 1 μM for domain 3, and >1 μM for domain 2, whereas stimulation of and subsequent Ca2+ influx through Ca2+ channels raise the cellular Ca2+ concentration from the basal level of ~100 nM to ~10 μM, suggesting that domain 3 has a pivotal role in Ca2+/CaM regulation of eEF1A.

著者

• BUNAI Fumihide

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Morita Kenya

  Graduate School of Life and Environmental Sciences, University of Tsukuba

関連論文

• Tetrahymena Eukaryotic Translation Elongation Factor 1A (eEF1A) Bundles Filamentous Actin through Dimer Formation
• Roles of Three Domains of Tetrahymena eEF1Ain Bundling F-actin
• ISOLATION OF FORMING-LIKE GENES IN TETRAHYMENA(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• THE ANALYSES OF THREE FUNCTIONAL DOMAINS OF ELONGATION FACTOR 1 α IN TETRAHYMENA(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)

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