Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.
スポンサーリンク
概要
- 論文の詳細を見る
Objective: The periodontal ligament (PDL) is a fibrous connective tissue composed of heterogeneous cell types, including PDL fibroblasts. It is not clear whether cells within the PDL fibroblast population retain the potency to differentiate into other cell types. Design: In the present study, clonal cell lines, derived from Clawn miniature swine PDLs, were established by gene transfection for a human telomerase reverse transcriptase, and characterized. Results: These cell lines, denoted TesPDL1–4, had PDL fibroblasts that showed fibroblastic morphology and expressed procollagen α1(I), osteopontin, periostin and alkaline phosphatase mRNA. Under the specific culture conditions, TesPDL3 cells also have the ability to express CD31, vascular endothelial cadherin, von Willebrand factor, osteocalcin, and to form extracellular mineralized nodules. Conclusions: Our data indicate that TesPDL3 cells have unique properties of expressing several phenotype of fibroblasts, vascular endothelial cells and osteoblasts in cultures.
- Elsevier Ltd.の論文
Elsevier Ltd. | 論文
- A real-time PCR method targeting a gene sequence encoding 16S rRNA processing protein, rimM, for detection and enumeration of Streptococcus thermophilus in dairy products
- A unimolecular nanocapsule : Encapsulation property of amphiphilic polymer based on hyperbranched polythreitol
- Capability-based egress network access control by using DNS server
- Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament
- Direct observations of sea-ice thickness and brine rejection off Sakhalin in the Sea of Okhotsk