INCORPORATION OF ^<15>N ADMINISTERED TO GERMFREE AND SPF PIGLETS AS ^<15>N-UREA INTO AMINO ACIDS OF HYDROLYZED LIVER AND MUSCLE PROTEINS

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^<15>N-urea was administered three times with a diet containing urea to two germfree and one specific pathogen free (SPF) piglets. They were killed on the fifth day after the last administration of ^<15>N-urea. The ^<15>N concentration in the amino acids isolated from the hydrolyzed trichloroacetic acid precipitates of the liver and muscle was determined. In SPF piglet, the ^<15>N was incorporated into all of the essential and nonessential amino acids, except threonine in both the liver and muscle and except histidine in the muscle. The ^<15>N concentration of each of the nonessential amino acids was higher than that of most of the essential amino acids in the liver and muscle. On the other hand, an excess of ^<15>N was not detected in the pooled essential and nonessential amino acids nor in the ammonia and glutamic acid in germfree piglets. It was concluded that the ammonia nitrogen converted from urea by the action of intestinal-bacterial urease in the gastrointestinal tract was utilized for the synthesis of all of the essential and nonessential amino acids, except threonine, in the pigs.
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